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Try adding slightly more bacteriostatic water

In Experiment 2, to investigate the effects of induced heat stress on oocyte quality and developmental competence, an in vitro model was developed

A., Bird, T

All three PRPP synthetase isoforms differ in kinetic and physical characteristics such as isoelectric points (pI), pH optima, activators and inhibitors
Be sure to talk to your doctor about using NAC in your protocol