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In addition, loss of glutathione peroxidase 4 (GPX4), a central regulator of ferroptosis, leads to hemolytic anemia and increases spleen erythroid progenitor death through receptor-interacting protein 3 (RIP3)-dependent necroptosis signaling 48
The assay is based on the conversion of citrate to isocitrate, to -ketoglutarate, which results in the production of NADPH
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