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One individual per group was euthanized before addressing the next animal in each group to render differences arising from the time of day at euthanization (e.g., cecal mass changes resulting from differences in time post-food consumption) comparable across all groups

Upon arrival, all samples were immediately ultracentrifuged for 10 min (Thermo Sorvall, Thermo Fisher Scientific, Waltham, MA, USA) at 1,000 g and 4C

PubMed Jastreboff, A.M., Aronne, L.J., Ahmad, N.N., Wharton, S., Connery, L., Alves, B., et al

In contrast, a true allergy tends to affect multiple organ systems within the body and tends to have a poorer outcome

The formation of glutathione begins with the conjugation of cysteine and glutamate, then glutathione synthase links this molecule with glycine