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Severe hypoglycemia was rare across all studies, but mild hypoglycemia was not uncommon and mostly associated with the use of sulfonylureas [17,19,76]

The GAPDH gene was served as the internal control ( CT method ( Cloning, expression and identification of rSmGST The first Spirometra GST gene deposited in GeneBank (AEI16476.1) was amplified by PCR with specific primers carrying BamHI and PstI restriction enzyme sites (underlined) (forward, 5-AT GGATCC ATGGGTTC GCTCCCGGTTC-3, and reverse, 5-AT CTGCAG CTAAGCATCACCACGCCAG-3), and the cycling protocol was as follows: 30 cycles of 95C for 50 s, 60C for 50 s and 72C for 50 s

3) starting with an image in which multiple endosomal puncta were visible, images were thresholded using the auto-selected threshold from the Triangle algorithm, and the same threshold was used for all images

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