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In practice, combining the cost of the skin punch biopsy, immunostaining, and specialist histopathological review might plausibly reach 200300 per sample, based on internal cost accounting in neuropathy research centers (though published examples are rare)
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Then, 1.0 mL of DPBS was added and cells were pelleted by centrifugation at 1200 rpm for 5 min at room temperature
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