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For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

This reproduction of the general features of 432 helicoid III suggests that the models can be used to study this helicoid theoretically and that they do not have to be perfect, but only need to resemble the helicoid shape sufficiently

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