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Cell viability was assessed using a Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the manufacturers instructions, with absorbance measured at 450 nm
All DPP-4 inhibitors are orally active, specific for DPP-4 and with a high affinity to the enzyme, although their binding characteristics and pharmacokinetic properties differ (61)
Do not wrap the site or put any pressure on it
Shekther et al
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