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However, while many people report positive results, the treatment remains medically debated in terms of long-term safety and effectiveness

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For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays

For extra hydration, a serum containing hyaluronic acid can be applied before the cream

Injection training: patients receive hands-on training on subcutaneous self-injection technique, vial storage (refrigerated), and reconstitution if supplied lyophilized